Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. 2003;89(2):31120. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. Hrabchak C, Flynn L, Woodhouse KA. ; Grishchenko, A.E. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. 2. Ann Surg Oncol. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. J. Phys. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. Chemical penetration enhancers for transdermal drug delivery systems. 2013;43(4):287303. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. Journal of Physical Chemistry, 58(11), 953-957. Naik A, Kalia YN, Guy RH. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. We observe that this maximum radius is below osmotic pressure or light scattering). The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. 2004;25(9):155967. Curley SA. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. By using this website, you agree to our All authors have consented to the submission of this manuscript for publication. In addition, we evaluated the regeneration of SC after microporation. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Carbohydrate Polymers, 36, 319-326. Carbohydrate Polymers, 68, 477-488. Rotureau, E.; Dellacherie, E.; Durand, A. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Trop J Pharm Res. 2012;9(2):14754. ; Dutt, N.V.K. Weissella: An Emerging Bacterium with Promising Health Benefits. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Kasaai, M. R.; Arul, J.; Charlet, G. (2000). On the molecular weight distribution of dextran T-500. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. The basic setup of a DLS instrument is shown in Figure 1. Federal government websites often end in .gov or .mil. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. This conjecture found to be correct. Ed., 9(2), 85-90. Advances in Physical Chemistry, Vol. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. WebFITC Products. ; Yevlampieva N.P. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Kasaai, Mohammad R. (2007). The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Carbohydr Polym. Drug Deliv. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. 4h after microporation, the sizes of the micropore decreased slightly. Arond L.H. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. 43-1, 115-147. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Colloid and Polymer Science, 286, 1223-1231. 10K, and c M.W. 2009;6(4):34354. Journal of Applied Polymer Science, 72, 871-876. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). Harding, S. E., & Simpkin, N. J. In fact, short chains do contain minor branching points. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Polymer molecular weight distribution. The solution was measured using an Anton Paar DMA35N densitometer. Epub 2021 Feb 9. (1999). The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. sharing sensitive information, make sure youre on a federal Unable to load your collection due to an error, Unable to load your delegates due to an error. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Carbohydrate Research, 160, 205-214. eCollection 2018 Feb. Molecules. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). Biomaterials, 22, 1891-1898. Sci. The effect of branching is best recognized from the molecular weight dependencies of []. NCI CPTC Antibody Characterization Program. Die Makromolekulare Chemie, 148(1), 229-238. Monolayers and Thin Films of Dextran Hydrophobically Modified. Ibrahim O, Munavalli GS, Dover JS. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Pharmacol Pharm. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Clipboard, Search History, and several other advanced features are temporarily unavailable. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. The site is secure. Proc. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm.